Nuclear Magnetic Resognance Practical
Purpose
- We took part for one day at a bigger research
- Recently it was found out that Oxygen can have regulatory functions
- Determine oxidation rates of TPH2's residues
- The protein:
MQPAMMMFSS KYWARRGFSL DSAVPEEHQL LGSSTLNKPN SGKNDDK
Setup
- We were split into 2 groups of 4 people
- We followed a researcher, who explained the process
- We kept any metal device away
Experiment
- We inserted the protein TPH2 in the magnet
- We measured relationship between the Hydrogen's nuclei number and the Nitrogen's nuclei number
- We insert \( H_2 O_2 \) into the protein solution, and we repeat the measurement
- During the measurement, the researcher set up the magnetic signals to prevent water's noise: water has a lot of hydrogen
Data Analysis
- We used the program CCPN Analysis to check which residues reacts with the oxygen
Conclusions
- Similar and close residues reacts in similar ways
MQPAMMMFSS KYWARRGFSL DSAVPEEHQL LGSSTLNKPN SGKNDDK